Peptide FAQ.

Peptide reconstitution is the process of mixing a lyophilized (freeze-dried) peptide with bacteriostatic water to create an injectable solution. The dry peptide ships in a sealed vial. You add a measured volume of BAC water through the rubber stopper, the powder dissolves into a clear solution at a known concentration, and the result is what you draw from for each dose.

The reconstitution calculator performs arithmetic on the values you enter. The math is deterministic: concentration = peptide / BAC water, volume to draw = dose / concentration, units = volume * 100 (for 100u syringes). If your inputs are correct, the outputs are exact.

Bacteriostatic water is sterile water mixed with 0.9% benzyl alcohol, an additive that suppresses bacterial growth in a punctured vial. It is the standard diluent for reconstituting lyophilized peptides because it lets you draw from the same vial multiple times over several weeks without contamination. Plain sterile water and saline lack this preservative.

There is no single right answer. Common choices are 1, 2, 3, or 5 mL. More BAC water produces a lower concentration, which makes small doses easier to draw accurately. Less BAC water produces a higher concentration, which makes the vial last longer. Two milliliters is a common starting volume for most 5 mg or 10 mg peptide vials.

You can, but only for single-use within a few hours. Plain sterile water and saline contain no preservative, so the vial is no longer protected against bacterial growth once punctured. For multi-dose vials that will be drawn from for days or weeks, bacteriostatic water is the correct diluent.

One milligram equals 1000 micrograms. Peptides labeled in milligrams (Semaglutide, Tirzepatide, BPC-157) are typically dosed in milligrams or fractions of a milligram. Peptides labeled in micrograms (Ipamorelin, CJC-1295, Tesamorelin) are often dosed in the 100 to 300 microgram range.

Concentrated vials produce small draw volumes. If a 5 mg vial is reconstituted in 1 mL of BAC water and you target a 0.25 mg dose, the draw is 0.05 mL, or 5 units on a 100 unit syringe. Adding more BAC water increases the draw volume and improves precision at the cost of taking longer to finish the vial.

Draws under 2 units on a 100 unit syringe are difficult to measure accurately because the scale becomes hard to read. The calculator surfaces this as an orange warning. The fix is usually to add more BAC water to the vial, which lowers concentration and increases the draw volume for the same dose.

GLP-1 titration is the practice of starting at a low dose of a GLP-1 receptor agonist (Semaglutide, Tirzepatide, Retatrutide) and increasing the dose over weeks. The slow ramp reduces gastrointestinal side effects such as nausea and lets the body adapt. A common Semaglutide schedule is 0.25 mg weekly for four weeks, then 0.5 mg, then 1.0 mg, then 1.7 mg, then 2.4 mg.

A standard 1 mL insulin syringe is marked in 100 units, where 100 units equals 1 mL. So 50 units equals 0.5 mL, 20 units equals 0.2 mL, and 10 units equals 0.1 mL. A 0.5 mL syringe is marked in 50 units. A 0.3 mL syringe is marked in 30 units.

All three are marked in insulin units (100 units per mL), but they differ in total capacity. A 1 mL syringe holds 100 units. A 0.5 mL syringe holds 50 units. A 0.3 mL syringe holds 30 units. Smaller syringes have a finer scale, which improves precision for small doses.

Most subcutaneous peptide injections use a 27 to 31 gauge needle, with 29 or 30 being typical. Lengths of 5/16 inch (8 mm) or 1/2 inch (12.7 mm) are common. The thinner the needle, the less injection discomfort.

Common subcutaneous injection sites are the abdomen (avoiding a 2-inch radius around the navel), the upper outer thigh, and the back of the upper arm. Rotate sites to avoid localized irritation or lipohypertrophy. The Peptide AI app supports site rotation tracking.

Most reconstituted peptides remain stable for two to four weeks when refrigerated at 2 to 8 degrees Celsius, kept upright, and shielded from light. Specific stability varies by peptide and by the concentration of benzyl alcohol in the diluent. Check the manufacturer's data sheet for your specific compound.

Most reconstituted peptides should not be frozen because freeze-thaw cycles can degrade the peptide and damage the structure. Lyophilized (unreconstituted) peptides can be frozen long-term. Once reconstituted, keep refrigerated and use within the stability window.

Semaglutide has a half-life of approximately 165 hours, or about 7 days. This long half-life is what allows once-weekly dosing. Steady state is reached after roughly 4 to 5 weeks at a given dose. See the full Semaglutide reference page.

Tirzepatide has a half-life of approximately 120 hours, or about 5 days. Like Semaglutide, this supports once-weekly dosing. Steady state is reached after roughly 4 weeks at a given dose. See the full Tirzepatide reference page.

BPC-157 (Body Protection Compound 157) is a synthetic peptide derived from a fragment of human gastric juice. It is studied in research contexts for soft tissue and tendon healing, gut lining repair, and reduction of inflammation. It is not approved by the FDA for human use. See the full BPC-157 reference page.

CJC-1295 with DAC (Drug Affinity Complex) has an added molecule that binds to serum albumin, extending the half-life from roughly 30 minutes to about 8 days. CJC-1295 without DAC clears quickly and is typically dosed multiple times daily, often paired with Ipamorelin. With-DAC supports once-weekly dosing but produces a sustained GH elevation rather than a pulse.

GHK-Cu is a copper peptide studied for skin remodeling, wound healing, hair density, and general tissue repair. It is used both topically (in serums) and subcutaneously in research contexts. Subcutaneous dosing is typically 1 to 3 mg per session.

Yes. The app is free to download on iOS and Android. Core tracking is included at no cost. AI insights, advanced biomarker analysis, and priority support require an upgrade ($6.99 weekly, $14.99 monthly, or $59.99 yearly).

iOS (iPhone and iPad, iOS 15 and later) and Android (Android 8.0 and later). The same account and data sync across both platforms.

Yes. Subscriptions are managed in App Store or Google Play subscription settings. Cancel anytime. You keep access to upgrade features until the end of the paid period.

No. We are not a vendor. We do not sell, prescribe, or recommend peptides. Peptide AI is a tracking and intelligence app. Users source compounds independently.

Apple Health on iOS and Health Connect on Android. The app reads sleep, HRV, weight, resting heart rate, and activity from these sources. Wearables that write to those platforms (Apple Watch, Oura, Whoop, Garmin, Samsung) are read indirectly.

The parser reads 36 biomarkers from any standard lab PDF, including IGF-1, ApoB, testosterone (total and free), HbA1c, hs-CRP, fasting insulin, fasting glucose, HDL, LDL, triglycerides, vitamin D, thyroid panel (TSH, T3, T4, free T3, free T4), liver enzymes (ALT, AST, GGT), cortisol, DHEA-S, and others.

The Peptide Score is a daily composite the app surfaces by combining sleep, HRV, weight, resting heart rate, and your dose log into a single number. It is designed to show whether the current protocol is moving the metrics you logged it to move.

User data is encrypted in transit and at rest. We do not sell or share user data. See the privacy policy at peptideai.co/privacy for full details.

No. Peptide AI and this site are educational and tracking tools. We do not prescribe, recommend, or evaluate whether any peptide is appropriate for any individual. Consult a licensed clinician for medical guidance.

Peptide AI was founded in February 2026 by Cameron Cook (CEO), with founding members Bennett Santus (CTO) and Sam Doyle (COO).