Peptide reference

Tesamorelin: Uses, Dosing, and Reconstitution Guide.

Tesamorelin is a stabilized synthetic analog of growth hormone-releasing hormone (GHRH) approved by the FDA under the brand name Egrifta, with a half-life of approximately 26 minutes and a once-daily subcutaneous dosing schedule. This page covers what it is, a worked reconstitution example, the standard protocol, storage, and FAQ.

Last updated: 2026-05-21
Overview

What is Tesamorelin.

Tesamorelin is a synthetic growth hormone-releasing hormone analog approved by the FDA for reducing excess visceral abdominal fat in HIV-associated lipodystrophy. It works by signaling the pituitary gland to produce more natural growth hormone. Tesamorelin is sold under the brand name Egrifta and is used off-label in longevity research.

Tesamorelin is a synthetic 44 amino acid analog of human growth hormone-releasing hormone (GHRH) with a single N-terminal modification (a trans-3-hexenoic acid group) that confers resistance to degradation by dipeptidyl peptidase-4 (DPP-4). The modification extends the half-life enough to support a clinically useful once-daily dose while preserving the natural pulsatile pattern of GH release from the pituitary somatotrophs. Like all GHRH analogs, Tesamorelin binds the GHRH receptor on the anterior pituitary and triggers endogenous GH release through the somatotroph axis.

Tesamorelin was approved by the FDA in 2010 under the brand name Egrifta for the reduction of excess visceral abdominal fat in HIV-infected patients with lipodystrophy. It remains the only FDA-approved GHRH analog and is the only peptide on this page list with a clinical approval as a peptide therapeutic. The visceral fat reduction effect was the basis for approval, mediated through GH-stimulated lipolysis of visceral adipose tissue.

The off-label and research use of Tesamorelin has expanded to include investigation of visceral adiposity in non-HIV populations, NAFLD, IGF-1 elevation in aging populations, and cognitive endpoints. Research-grade Tesamorelin is sold for laboratory use and is not approved for human consumption outside the approved indication.

Research applications

Common research applications.

Reconstitution

Typical reconstitution.

The approved Egrifta product is reconstituted with sterile water at the time of injection and used immediately. For research-grade Tesamorelin reconstituted with bacteriostatic water for multi-day storage, the worked example below uses a 5 mg vial and 2 mL of BAC water. Use the reconstitution calculator for different vial sizes.

InputValueNotes
Vial size5 mg (5000 mcg)Lyophilized powder
BAC water added2 mLBacteriostatic water with 0.9% benzyl alcohol
Resulting concentration2.5 mg / mL5 mg divided by 2 mL
Approved dose1 mg (1000 mcg)Once daily
Volume to draw at 1 mg0.40 mL1 mg divided by 2.5 mg / mL
Units on 1 mL (100 unit) syringe40 units0.40 mL multiplied by 100
Volume to draw at 2 mg0.80 mL (80 units)Higher research dose
Doses per vial at 1 mg5 dosesAbout 5 days per vial at the approved dose
Protocols

Common protocols.

The table below summarizes the approved protocol and common research variants. This is reference information for research contexts only and is not a recommendation for human use beyond the approved indication.

ProtocolDoseFrequencyDuration
Approved (Egrifta)1 mg (1000 mcg)Once daily, subcutaneous abdomenContinuous, with periodic re-evaluation
Standard research1 mgOnce daily, evening12 to 26 weeks
Cycled research1 mgOnce daily, 5 days on / 2 days off12 weeks then taper
Higher dose research2 mgOnce daily8 weeks maximum (IGF-1 monitoring)
Half-life and frequency

Half-life and dosing frequency.

Tesamorelin has a plasma half-life of approximately 26 minutes after subcutaneous injection. The N-terminal trans-3-hexenoic acid modification provides resistance to DPP-4, which extends the half-life relative to unmodified GHRH 1 to 44 (which clears in minutes). The half-life is intentionally short: it preserves the natural pulsatile pattern of GH release without producing a sustained, non-pulsatile elevation that would risk pituitary desensitization.

Once-daily subcutaneous dosing is the standard, typically given in the evening to align with the natural overnight GH pulse during deep sleep. Steady state IGF-1 elevation is reached after roughly 4 weeks at a given dose; visceral fat reduction is detectable at 12 to 26 weeks in published trials.

Storage

Storage and handling.

Lyophilized Tesamorelin is stable for months at 2 to 8 degrees Celsius and for years at -20 degrees Celsius. Keep the vial sealed and shielded from light.

Reconstituted Tesamorelin is typically stable for 7 to 14 days when refrigerated at 2 to 8 degrees Celsius, kept upright, and shielded from light. The approved Egrifta product specifically calls for use within hours of reconstitution with sterile water; bacteriostatic water extends the stability window. Do not freeze. Discard if the solution becomes cloudy.

Swirl gently to dissolve. Do not shake.

FAQ

Frequently asked questions.

What is the half-life of Tesamorelin?
Tesamorelin has a plasma half-life of approximately 26 minutes after subcutaneous injection. The short half-life is intentional: it preserves the natural pulsatile pattern of GH release from the pituitary while resisting DPP-4 degradation longer than native GHRH.
How do I reconstitute a 5 mg Tesamorelin vial?
A common reconstitution for a 5 mg Tesamorelin vial is 2 mL of bacteriostatic water. That produces a concentration of 2.5 mg per mL. A 1 mg dose is 0.4 mL or 40 units. A 2 mg dose is 0.8 mL or 80 units. Use the reconstitution calculator to re-run the math.
What is Tesamorelin used for?
Tesamorelin is a stabilized synthetic analog of GHRH approved by the FDA under the brand name Egrifta for the reduction of excess visceral abdominal fat in HIV-infected patients with lipodystrophy. It is also studied in research contexts for visceral adiposity, NAFLD, IGF-1 elevation, and cognitive endpoints in aging populations.
What is the typical Tesamorelin dose?
The approved dose is 1 mg (1000 mcg) once daily by subcutaneous injection, typically in the abdomen and rotated between sites. Most research protocols mirror the approved dose.
How is Tesamorelin different from Sermorelin and CJC-1295?
All three are GHRH-related analogs that bind the GHRH receptor on the anterior pituitary. Sermorelin is the unmodified 1 to 29 fragment with a 10 to 20 minute half-life. Tesamorelin is a stabilized form with a 26 minute half-life. CJC-1295 (no DAC) has a 30 minute half-life. Tesamorelin is the only one of the three with FDA approval.
How long does reconstituted Tesamorelin last in the fridge?
Reconstituted Tesamorelin is typically stable for 7 to 14 days when refrigerated at 2 to 8 degrees Celsius, kept upright, and shielded from light. The approved product specifically calls for use within hours of reconstitution with sterile water; bacteriostatic water extends the window.
Does Tesamorelin raise IGF-1?
Yes. Tesamorelin produces a dose-dependent elevation of plasma IGF-1, the primary downstream biomarker of GHRH-stimulated GH release. Published clinical trial data shows IGF-1 increases of 50 to 100% from baseline at the approved 1 mg daily dose over 26 weeks.
Disclaimer. This page is an educational reference. Nothing here is medical advice. Peptides sold as research chemicals are not for human consumption. Tesamorelin is FDA-approved only for HIV-associated lipodystrophy; all other uses are off-label. Consult a licensed clinician before starting, changing, or stopping any therapy.
Related

Related references.

Related peptide

Sermorelin

Unmodified 1 to 29 GHRH fragment. The original of the class.

Read reference →
Related peptide

CJC-1295

Another stabilized GHRH analog, in two forms.

Read reference →
Tool

Reconstitution Calculator

Run your own vial size and BAC water volume through the calculator.

Open calculator →
Article

GHRH analogs compared

Sermorelin, Tesamorelin, and CJC-1295 side by side.

Read article →

Track Tesamorelin in the app.

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