Peptide reference

Sermorelin: Uses, Dosing, and Reconstitution Guide.

Sermorelin is the synthetic 1 to 29 N-terminal fragment of endogenous human GHRH, with a half-life of approximately 10 to 20 minutes, dosed nightly by subcutaneous injection. This page covers what it is, a worked reconstitution example, typical research protocols, storage, and the most common follow-up questions.

Last updated: 2026-05-21
Overview

What is Sermorelin.

Sermorelin is a synthetic fragment of growth hormone-releasing hormone (GHRH). It signals the pituitary gland to release the body's own growth hormone in natural pulses, preserving the pituitary axis. Sermorelin is FDA approved for pediatric growth hormone deficiency and is used off-label in adult longevity protocols. Half-life is short, around 10 to 20 minutes.

Sermorelin is a synthetic 29 amino acid peptide that reproduces the N-terminal 1 to 29 fragment of endogenous human growth hormone-releasing hormone (GHRH). The 1 to 29 fragment retains the full biological activity of the parent 44 amino acid GHRH and is the smallest sequence that still binds and activates the GHRH receptor on the anterior pituitary. The molecule is unmodified, meaning it does not carry the stabilizing substitutions or albumin-binding tails that distinguish later analogs such as Tesamorelin and CJC-1295.

Mechanistically, Sermorelin binds the GHRH receptor on pituitary somatotrophs and triggers a short, sharp pulse of endogenous GH release. The pulse pattern mimics what the body's own GHRH does, which is the appeal of the molecule: it preserves the natural pulsatile signaling without producing the sustained, non-pulsatile elevation associated with some longer-acting analogs.

Sermorelin was historically marketed in the United States under the brand name Geref for the diagnosis and treatment of pediatric growth hormone deficiency; the branded product was discontinued in 2008 for commercial reasons. The molecule remains available through compounding pharmacies and as a research chemical. It is not currently FDA-approved as a finished pharmaceutical product.

Research applications

Common research applications.

Reconstitution

Typical reconstitution.

The worked example below uses a 5 mg vial and 2 mL of bacteriostatic water. Adjust the BAC water volume in the reconstitution calculator for different vial sizes.

InputValueNotes
Vial size5 mg (5000 mcg)Lyophilized powder
BAC water added2 mLBacteriostatic water with 0.9% benzyl alcohol
Resulting concentration2.5 mg / mL (2500 mcg / mL)5 mg divided by 2 mL
Common dose200 mcgOnce nightly
Volume to draw at 200 mcg0.08 mL200 mcg divided by 2500 mcg / mL
Units on 1 mL (100 unit) syringe8 units0.08 mL multiplied by 100
Volume to draw at 300 mcg0.12 mL (12 units)Higher single-dose protocol
Doses per vial at 200 mcg25 doses5000 mcg divided by 200 mcg
Protocols

Common protocols.

The table below summarizes typical research and compounding dosing patterns. This is reference information for research contexts only and is not a recommendation for human use.

ProtocolPer doseFrequencyCycle length
Low nightly200 mcgOnce nightly before bed12 to 24 weeks
Standard nightly300 mcgOnce nightly before bed12 to 24 weeks
Higher nightly500 mcgOnce nightly before bed12 weeks
5 days on / 2 off300 mcgNightly, 5 days per week12 to 24 weeks
Half-life and frequency

Half-life and dosing frequency.

Sermorelin has a very short plasma half-life of approximately 10 to 20 minutes. It is the unmodified 1 to 29 fragment of GHRH and is rapidly degraded by DPP-4 and cleared via renal filtration. The molecule does not carry stabilizing modifications.

The short half-life is what produces the single sharp GH pulse and what dictates the nightly dosing schedule. A dose given before bed amplifies the natural GH pulse that occurs during deep sleep. Daytime dosing produces a pulse but with smaller aggregate effects on IGF-1 due to background hormonal context. Some 5 days on / 2 days off protocols are used to limit any potential downregulation, although evidence for downregulation at typical nightly doses is limited.

Storage

Storage and handling.

Lyophilized Sermorelin is stable for months at 2 to 8 degrees Celsius and for years at -20 degrees Celsius. Keep the vial sealed and shielded from light.

Reconstituted Sermorelin is typically stable for 2 to 3 weeks when refrigerated at 2 to 8 degrees Celsius, kept upright, and shielded from light. Do not freeze. Discard if the solution becomes cloudy, discolored, or develops visible particulates.

Swirl gently to dissolve. Do not shake.

FAQ

Frequently asked questions.

What is the half-life of Sermorelin?
Sermorelin has a very short plasma half-life of approximately 10 to 20 minutes. It is the unmodified N-terminal 1 to 29 fragment of native GHRH and clears the plasma quickly via DPP-4 degradation and renal filtration. The short half-life produces a single sharp GH pulse rather than a sustained elevation.
How do I reconstitute a 5 mg Sermorelin vial?
A common reconstitution for a 5 mg Sermorelin vial is 2 mL of bacteriostatic water. That produces a concentration of 2500 mcg per mL. A 200 mcg dose is 0.08 mL or 8 units. A 300 mcg dose is 0.12 mL or 12 units. Use the reconstitution calculator to re-run the math.
What is Sermorelin used for?
Sermorelin is the 1 to 29 N-terminal fragment of human GHRH and is studied for triggering a natural GH pulse from the anterior pituitary. Historical use included pediatric growth hormone deficiency. Current research and compounding use includes age-related GH decline, body composition, and sleep endpoints.
What is the typical Sermorelin dose?
Common research and compounding dosing is 200 to 500 micrograms once nightly before bed, given subcutaneously. The nightly timing aligns the dose-triggered GH pulse with the natural GH pulse during deep sleep.
How is Sermorelin different from CJC-1295 and Tesamorelin?
All three are GHRH-related. Sermorelin is the unmodified 1 to 29 fragment with a 10 to 20 minute half-life. Tesamorelin is a stabilized 1 to 44 analog with a 26 minute half-life and is FDA-approved. CJC-1295 (no DAC) is a stabilized 1 to 29 analog with a 30 minute half-life.
How long does reconstituted Sermorelin last in the fridge?
Reconstituted Sermorelin is typically stable for 2 to 3 weeks when refrigerated at 2 to 8 degrees Celsius, kept upright, and shielded from light. Discard if the solution becomes cloudy.
Why dose Sermorelin at night?
Endogenous GH is released in pulses, with the largest pulse occurring shortly after sleep onset during the deep slow-wave sleep stage. A Sermorelin dose given before bed amplifies the size of that natural pulse. Daytime dosing produces a pulse but at a time when endogenous GH activity is at baseline.
Disclaimer. This page is an educational reference. Nothing here is medical advice. Peptides sold as research chemicals are not for human consumption. Sermorelin is not currently FDA-approved as a finished pharmaceutical product. Consult a licensed clinician before starting, changing, or stopping any therapy.
Related

Related references.

Related peptide

Tesamorelin

The stabilized GHRH analog with FDA approval.

Read reference →
Related peptide

CJC-1295

Another stabilized 1 to 29 analog, with or without DAC.

Read reference →
Tool

Reconstitution Calculator

Run your own vial size and BAC water volume through the calculator.

Open calculator →
Article

GHRH analogs compared

Sermorelin, Tesamorelin, and CJC-1295 side by side.

Read article →

Track Sermorelin in the app.

Peptide AI Stack Intelligence is free on iOS and Android. Log every dose, track IGF-1, watch sleep quality.