MOTS-c Reconstitution
How to mix MOTS-c and read the exact units to draw. Enter your vial size and bacteriostatic water below for the concentration and dose on a U-100 insulin syringe.
How to reconstitute MOTS-c
- Swab the rubber stopper of the MOTS-c vial and the bacteriostatic water vial with an alcohol wipe.
- Draw your chosen volume of bacteriostatic water into a syringe (1 to 3 ml is common).
- Inject the water slowly down the inside wall of the MOTS-c vial, not directly onto the powder.
- Swirl gently until the powder fully dissolves. Do not shake. Shaking can damage the peptide.
- Refrigerate and use within about 4 to 6 weeks.
MOTS-c concentration by water volume
For a 5 mg vial, the concentration depends only on how much bacteriostatic water you add. More water lowers the concentration and makes small doses easier to measure.
| BAC water added | Concentration | Per 1 unit |
|---|---|---|
| 1 ml | 5,000 mcg/ml | 50 mcg |
| 2 ml | 2,500 mcg/ml | 25 mcg |
| 3 ml | 1,666.7 mcg/ml | 16.7 mcg |
MOTS-c units to draw
At 2,500 mcg/ml (a 5 mg vial in 2 ml of bacteriostatic water), here is the draw for common doses on a U-100 insulin syringe. Change any number in the calculator above for your own vial.
| Dose | Units to draw | Volume |
|---|---|---|
| 100 mcg | 4 units | 0.04 ml |
| 200 mcg | 8 units | 0.08 ml |
| 250 mcg | 10 units | 0.10 ml |
| 300 mcg | 12 units | 0.12 ml |
| 500 mcg | 20 units | 0.20 ml |
MOTS-c dosing
Primarily animal-model data; community dosing protocols exist but aren't clinically validated. Enter your own vial size, water volume, and target dose in the calculator above to convert any MOTS-c protocol into exact insulin-syringe units. For the full profile, see the MOTS-c compound page.
Storing reconstituted MOTS-c
Once mixed, keep MOTS-c refrigerated at 2 to 8 degrees C and use it within about 4 to 6 weeks. Store unopened lyophilised powder in the freezer. Protect from light and avoid repeated freeze-thaw cycles, which degrade the peptide.
The math
- Concentration (mcg/ml) = vial size in mg × 1,000 ÷ bacteriostatic water in ml.
- Volume for your dose (ml) = dose in mcg ÷ concentration.
- Units to draw = volume in ml × 100 on a U-100 insulin syringe.
Prefer a full workspace? The peptide dosage & reconstitution calculator adds blend, converter, half-life, and titration tools.
MOTS-c reconstitution FAQ
Draw bacteriostatic water into a syringe, inject it slowly down the inside wall of the MOTS-c vial, and swirl gently until the powder dissolves. Do not shake. The concentration is the vial size in mg times 1,000, divided by the millilitres of water you added.
There is no single correct amount. 1 to 3 ml per vial is common. More water makes small doses easier to measure. A 5 mg vial in 2 ml gives 2,500 mcg/ml. The calculator shows the concentration for any volume you choose.
Units to draw equals your dose in mcg divided by the concentration in mcg/ml, times 100 on a U-100 insulin syringe. At 2,500 mcg/ml, a 250 mcg dose is 10 units.
Keep reconstituted MOTS-c refrigerated at 2 to 8 degrees C and use it within about 4 to 6 weeks. Store the unopened lyophilised powder in the freezer. Protect from light and avoid repeated freeze-thaw.
Track MOTS-c in Peptide AI
Log your doses, connect labs and wearables, and let the AI show you how MOTS-c is affecting your biomarkers over time.
Download the appEducational information only. This is not medical advice. Peptide AI is an informational and tracking platform. Everything on this page is for general education. It is not a substitute for professional medical advice and is not a recommendation to use any compound. Many peptides listed here are research compounds the FDA has not approved for human use. Talk to a qualified, licensed healthcare provider before you start, change, or stop any protocol.